Fig. 4. CME was only supported in cells expressing functional triskelia. (A) Representative confocal micrographs of transferrin uptake in cells expressing some of the CHC constructs on a CHC knockdown background. Panels show GFP (left), transferrin-Alexa-Fluor-546 (middle) and merge (right). Bar, 20 µm. (B) Quantification of transferrin uptake. Each cell was outlined using the GFP channel as a guide, the transferrin-Alexa-Fluor-546 image was then thresholded and the number of puncta within the ROI was counted. Results are mean ± s.e.m. of 18-38 cells per construct, normalised to control; ^**P<0.01 compared with transferrin uptake in GFP cells.