Fig. 6. Rescue of mitotic defects by trimeric CHC constructs. Quantification of (A) mitotic index and (B) the frequency of metaphase-like cells with misaligned chromosomes in cells expressing CHC constructs on a CHC-knockdown background. Mitotic index was assessed by counting the number of GFP-positive cells in mitosis as a proportion of the total number of GFP-positive cells, per unit area. The number of GFP-positive cells in a metaphase-like state with misaligned chromosomes was also counted. Hoechst 33342 staining was used to identify mitotic cells and misaligned chromosomes. Results are mean ± s.e.m.; ^**P<0.01 compared with levels in GFP cells.