Fig. 2. H⁺-ATPase and protein disulfide isomerase are not delivered to phagocytic cups. Cells expressing CRAC-GFP (green) were allowed to engulf yeast particles (red) for 10 minutes. The cells where then fixed and processed for total immunofluorescence to detect H⁺-ATPase (A) or protein disulfide isomerase (PDI) (B) H⁺-ATPase (white) was detected in maturing early phagosomes but not in phagocytic cups (data not shown) or in newly formed phagosomes. Protein Disulfide Isomerase (white) was not detected in newly formed or maturing early phagosomes. Bars, 4 µm.