Fig. 8. Absence of massive vesicular transport around phagocytic cups. Cells were allowed to engulf yeast particles for ten minutes, then fixed and processed for electron microscopy. The left panel is a representative image of a cell (C) with a phagocytic cup containing a yeast particle (Y). The right panel is a magnification of the box in the left panel. The phagocytic cup is surrounded by a continuous layer of F-actin (arrows) and presents no evidence for fusion or fission of vesicles. An endoplasmic reticulum cistern (arrowheads) is visible in the vicinity of the phagocytic cup but no direct contact was observed. Bar, 0.5 µm.