Fig. 6. (A) Representative image of cytoplasmic DAF-16::GFP localization in an L1 worm. (B) Representative image of intermediate DAF-16::GFP localization in an L1 worm showing both cytoplasmic and nuclear localization. (C) Representative image of nuclear DAF-16::GFP localization in an L1 worm. (D) DAF-16::GFP in daf-16(mu86) (labeled WT) and DAF-16::GFP in ifta-2(tm1724);daf-16(mu86) [labeled ifta-2(tm1724)] were compared for nuclear vs cytoplasmic DAF-16::GFP localization. Individual worms were classified as cytoplasmic, intermediate or nuclear by three researchers. Numbers are the average (to the nearest whole number) of the scores of three researchers. The proportion of nuclear, intermediate and cytoplasmic DAF-16::GFP localization for each strain was compared by the Chi² test. ^*P<0.001, Chi²=9.2475.