Fig. 11. ER domains required for PRL array targeting. (A) Schematic of ER functional domains with residue coordinates of the deletion mutants used in these experiments. (B) Representative images of GFP-ER₂₈₂, GFP-ER₅₃₄ and GFP-ER₅₅₄ colocalized with endogenous RNAPII or acetylated histone H3 by immunofluorescence. Each truncation was transiently expressed in PRL-HeLa cells and treated with vehicle or E2 (10 nM, 2 hrs) as indicated. GFP-X refers to the GFP signal captured from each receptor. DAPI indicates counterstained nuclei. (C) Comparison of GFP-ER and GFP-ER truncation mobility within the nucleoplasm or the array. PRL-HeLa cells were treated with vehicle or E2. Bulk denotes recovery times in the nucleoplasm and array identifies recovery times at the PRL array. Half-time of recovery was determined and is shown as a bar graph (see Materials and Methods). n=30 for FRAP analysis under each condition,