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Figure 3


Fig. 3. Real-time visualization of alterations in chromatin structure of the GFP-ER-targeted PRL array in response to E2 or 4HT. Time-lapse images were obtained by deconvolution microscopy using live cells transiently expressing GFP-ER. Ligand addition is indicated after t=0, and frames are labeled with time points after t=0. The images were deconvolved and those shown are projected image stacks. (A) Typical data from PRL-HeLa cells treated with E2. (B) Data from PRL-HeLa cells treated with 4HT. n=10 for cells analyzed. The size bar indicates length in microns.