Fig. 1. HspA12B whole-mount in situ hybridization at different zebrafish developmental stages. (A-G) Middle somite stages. (A-C) Dorsal views of head, trunk and tail, respectively, showing staining in ventral hematopoietic and vasculogenic mesoderm. (D,E) Lateral (D) and enlarged lateral view (E) of head region showing expression in rhombomeres. (F) Lateral view showing expression in first somite. (G) Dorsal view of ear showing staining in the anterior part of the otic vesicle. (H-J) Embryos at 24 hpf, dorsal view of head region (H), lateral view of the whole embryo (I), JB4 cross section in the truck region of embryos at 24 hpf (J). Arrows point to arterial and venous structures. NT, neural tube; NO, notochord. (K-N) Embryos at 36 hpf. Enlarged lateral view of the head (K), front view showing staining in the heart (arrow) (L), dorsal view of the head region (M); arrow indicates duct of Cuvier. Lateral view of trunk (N) showing staining in both axial vessels and ISVs. (O) Lateral view of an embryo at 48 hpf.