Fig. 2. Knockdown of HspA12B by MOs during zebrafish development. (A) In vitro transcription and translation in the absence of MOATG or in the presence of various concentrations of MOATG or MM, demonstrating the efficiency of MOATG in blocking the translation of HspA12B. (B) RT-PCR of zebrafish HspA12B from zebrafish embryos injected with various amounts of MOs3rd. (C-E) Embryos at 48 hpf injected with (C) 1.0 mM MM, (D) 1.0 mM or (E) 2.0 mM MOATG. (F) Percentage of normal circulation in morphants injected with 1.0 mM MOATG or MM at 26 hpf. Data are presented as the mean ± s.e.m. of three or more independent experiments; the difference between MM and MOATG was significant (P<0.01) as accessed by two tailed Student's t-test.