Fig. 5. Expression profile of human HspA12A and HspA12B in human cell lines. (A) Detection of HspA12B mRNA in human cell lines by northern blot. Signal is evident only in the HUVEC lane (upper panel), 28S and 18S staining is shown as equal loading control (lower panel). (B) Comparison of HspA12A and HspA12B mRNA levels in cell lines using real-time PCR. Data from two independent experiments were normalized with the expression levels in HUVECs and are presented as the mean ± s.e.m.; the difference of expression levels between HUVECs and other cell lines were all significant (P<0.05) as tested by two tailed Student's t-test.