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Figure 6


Fig. 6. Knockdown and overexpression of HspA12B. (A) HEK 293 cells cultured in six-well plate were transfected with 0.1 µg pCS2+-HspA12B-Flag and various siRNAs at 62.5 nM final concentration. Cell lysates were collected after 48 hours and the expression level of HspA12B-Flag was checked by western blot using anti-FLAG antibody. P, pool of si1, si2, si3 and si4. (B) GAPDH loading control for (A). (C) HUVECs were transfected with si1 and si3 at 62.5 nM final concentration in six-well plates and endogenous HspA12B expression was examined after 48 hours by western blot using Ab4112 antibody; Lane 4 was loaded with lysate from HUVECs infected with HspA12B-C-FLAG adenovirus. (D) GAPDH loading control for (C).