Fig. 8. Wound healing assays using HUVECs. (A,B) HUVECs infected with empty control and sense adenoviruses were grown to confluence. Wounds were made using 200 µl pipette tips in the cell lawn 48 hours after infection and media was changed to EBM-2 supplemented with 1% serum after the injury. Pictures were taken at 0 and 48 hours. (C,D) HUVECs infected with the same adenoviruses were grown to confluence. After making the wound, EBM-2 supplemented with 1% serum and VEGF (10 ng/ml) was added. Pictures were taken at 0 and 30 hours post injury. (E,F) HUVECs transfected with NC, si1 and si3 were grown to confluence and wounds were made in the cell monolayer 48 hours after transfection. EBM-2 supplemented with 1% serum and VEGF (10 ng/ml) was added. Pictures were taken at 0 and 24 hours post injury. Representative pictures were shown from at least three independent experiments.