Fig. 7. Silencing Pfn1 alters the localization of VASP in migrating HUVECs. In control cells (denoted by `C'), VASP is predominantly localized at the focal adhesions and actin stress-fibers, and has a punctate distribution at the leading edge. Pfn1-deficient cells (denoted by `P') have much stronger localization of VASP at the leading edge. The inset in each panel shows a magnified view of the boxed region depicting differences in VASP localization at the leading edge between the two experimental conditions. Bar, 10 µm.