Fig. 2. PAIR2 accumulation in the meiocyte nucleus is induced following pre-meiotic DNA synthesis. (A) Immunofluorescence of incorporated BrdUs and PAIR2 proteins against pre-meiotic PMCs. Staining pattern of BrdU (magenta) and PAIR2 (green) was divided into five categories: (1) neither a BrdU nor a PAIR2 signal; (2) faint BrdU but no PAIR2 signal; (3) intense BrdU and faint PAIR2 signals; (4) faint BrdU and intense PAIR2 signals; and (5) no BrdU and filamentous PAIR2 signals. Chromosomes were counter-stained with DAPI (blue). Bar, 5 µm. (B) Frequency of each category of meiocyte in anthers 0.3, 0.4 and 0.5 mm long. The numbers above each bar correspond to each category of the meiocytes in A.