Fig. 4. PAIR2 localizes on the meiotic chromosome axis and delocalizes after synapsis is completed in zygotene and pachytene. (A) Spread chromosomes (red) and PAIR2 localization (green) in the late zygotene meiocyte with destructed nucleus. (B) A magnified image of a box in A. (C) Another magnified image of early pachytene chromosomes. Arrows in B and C indicate synapsed regions of homologous chromosomes, and arrowheads show unsynapsed regions. (D) Spread chromosomes in pachytene. Bars, 5 µm.