Fig. 5. EM observation of rice meiotic chromosomes. (A) Immunogold localization of anti-PAIR2 antibody in an ultrathin section of a wild-type meiocyte at zygotene. Arrows indicate the electron-dense and filamentous structure observed in the nucleus. Nu, nucleolus. Bar, 1 µm. (B) Magnified view of the box in A. Bar, 0.2 µm. (C) A negative control of A, excluding primary antibody. Bar, 1 µm. (D) EM observation of pair2 mutant meiocytes. Filamentous and electron-dense chromosome axes (arrows) were observed to be the same as in the wild type (not shown). Bar, 0.2 µm.