Fig. 6. (A) Schematic diagram of C. elegans titin protein (Ce-titin) showing the PEVT domain and polypeptide antigens used to raise antibodies EU145, EU102 and EU143 (grey boxes). (B) Adult C. elegans stained by indirect immunofluorescence for endogenous Ce-titin using affinity-purified EU102, EU145 and EU143 antibodies. All three gave typical striated staining in the muscle myofilament lattice. (C) Indirect immunofluorescence staining for endogenous Ce-titin (R, red; Cy3) and Ce-lamin (G, green; FITC) in wild-type embryos stained with affinity-purified EU145 or EU143 antibodies. DNA was stained with DAPI. Ce-lamin was detected with rabbit serum 3932 (Gruenbaum et al., 2002). (D) Indirect immunofluorescence staining for endogenous Ce-titin using rabbit polyclonal EU102 (G, green; FITC) and Ce-lamin rat serum 3933 (R, red; Cy3). DNA was stained with DAPI in wild-type (WT) or lmn-1(RNAi) embryos. Bars, 5 µm.