Fig. 10. Suppression of Beclin expression does not affect the growth rate or clonogenicity of U-251 cells. (A) Control (solid line) and Beclin KD (dashed line) cells were seeded in 60 mm dishes on day 0 at an initial density of 200,000 cells per dish. On each of the indicated days, cells were harvested from three dishes in each group and counted with a Coulter Z1 particle counter (mean ± s.e.). (B) Control or Beclin KD cells were seeded in soft agar and assayed for colony formation after 14 days as described in the Materials and Methods. Results are means (± s.e.) of colony counts performed on three dishes.