Fig. 4. Loss of E-cadherin increases II-4 cell attachment to extracellular matrix proteins. 2D cultures of pBabe-, H-2k^d-EcadC25- or H-2k^d-Ecad-II-4 cells were trypsinized briefly and replated onto plates coated with either Type I collagen (A), Type IV collagen (B), laminin-1 (C) or fibronectin (D) for 20 minutes. Attached cells were quantified by optical density determined at 590 nm. Results are calculated as the mean ± s.d. of four replicates and experiments were repeated four times. P<0.01 for H-2k^d-Ecad-II-4 cells vs control cells in adherence to Types I and IV collagen substrates. P<0.05 for H-2k^d-Ecad-II-4 cells vs control cells in adherence to laminin-1 and fibronectin substrates.