Fig. 5. Determination of nuclear position in wild-type and mutant shmoos. The positions of nuclei in shmoos were classified into three groups: (i) in/at the shmoo neck (top), (ii) in the centre of the cell (centre) or (iii) opposite the mating projection (bottom). The percentage of cells in each class was calculated. n>250 shmoos for each strain. (A) Wild-type (HZH638) and mdy2 mutant strains (HZH686) were induced to form shmoos by treatment with -factor (5 µM) for 2.5 hours. Cells were fixed, stained with DAPI, and then scored for nuclear position. (B) Cultures of wild-type (W303-1A) and mdy2 mutant (HZH686) strains harbouring pGAL-GFP-NUP116 plasmids were grown to log phase and induced in 3% raffinose and 1% galactose medium for 2 hours, and then 5 µM -factor was added for another 3 hours. After brief fixation, wild-type (WT; W303-1A) and mdy2 cells were scored for GFP-Nup116 localization (n>300).