Fig. 6. SBDS-GFP localizes to the pseudopods of cells treated in a perfusion chamber with a constant concentration of chemoattractant (cAMP). (A,B) Cells were perfused before imaging with (A) buffer in the absence of cAMP for 10 minutes or (B,C) buffer containing 5x10^–8 M cAMP for 10 minutes; a, anterior end; u, uropod. Bar, 5 µm.