Fig. 3. Constraint of chromatin dynamics with differentiation restricts changes in nuclear organization. (A) An eye imaginal disc expressing GFP (pink) in differentiating cells present posterior to the morphogenetic furrow. There is a gradient of differentiation, with cells further away from the furrow having started differentiating earlier than those present closer to the furrow. A series of images of the posterior end of the disc were taken (green box). (B) A projected image representing the area demarcated by the green box is shown. Each image was divided into two halves, categorizing the nuclei into early differentiating and late-differentiating nuclei. (C) Box plots of distances between the mRFP signal from the tagged bw^D locus and the closest CIDGFP dot was measured from projected images and corrected by the nuclear radius. No significant difference in the distribution was observed in the late-differentiating cells as compared with the early differentiating cells.