Fig. 5. bw^D can get closer to the centromere than bw⁺. (A) Histograms of all the 3D measurements from all the movies in this study with t= 30 seconds and 3 minutes suggest that the heterochromatic insertion in bw^D allows it to come into close proximity (<1.0 µm) to centric heterochromatin. (B,C) Traces showing the distances between mRFP-CID at each time point in individual movies (each movie a different color trace). Movement of the tagged bw region in undifferentiated nuclei of the eye imaginal discs was followed for 1-2 hours. Images were taken every 3 minutes. Image analysis was done in 3D. Nuclei were divided into the three categories. The first category contains nuclei where the distance between CIDGFP and mRFP at t=0 was >2.0 µm, the second category contains nuclei where the distance was between 1.0 and 2.0 µm, and the third category contains nuclei where the distance was <1.0 µm. When the distance between the centromere and the bw^D locus is >2.0 µm the fluctuation in distances between the two loci is more apparent (left panel), as compared with the change in distance when the relative distance between bw^D and the closest centromere is <2.0 µm. (B) Traces from movies of wild-type nuclei. (C) Traces from movies of bw^D nuclei. (D) Graph of the distance between the two closest CIDGFP dots in six nuclei. These centromeres do not experience any large-scale movements.