Fig. 2. Expression of ZnT-8 in human islets at the protein level. (A) Western blot with anti-human ZnT-8 antibody showing the presence of the protein in total extracts of human pancreas. Lane 1, HeLa ZnT-8-EGFP (positive control); lane 2, HeLa-EGFP (negative control); lane 3, rat insulinoma INS-1E cells; lane 4, human pancreatic islet extracts. (B) The ZnT-8 protein is detected (red) by immunochemistry of pancreatic islet cells. Nuclei were counterstained with Carrazi's hematoxylin. Bar, 30 µm.