JCS -- Phelan et al. 119 (20): 4225 Figure IG3

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Fig. 3. Trafficking of GFP-CPS, UbGFP-CPS and GFP-Sna3p in vps27 ${Delta}$ , vps4 ${Delta}$ and fab1 ${Delta}$ /vps4 ${Delta}$ cells. Cells were transformed with plasmids for expressing GFP-CPS, UbGFP-CPS or GFP-Sna3p, grown to mid-exponential phase and inspected by light and fluorescence microscopy. In all three mutants cargo proteins predominantly localize to the swollen MVB characteristic of class-E mutants. Thus, unlike in AP-1 complex component and fab1 mutants, UbGFP-CPS and GFP-Sna3p do not traffic to the vacuole lumen in vps27 ${Delta}$ , vps4 ${Delta}$ and fab1 ${Delta}$ /vps4 ${Delta}$ cells.