Fig. 3. Depleting Dab2 alone inhibits LDLR endocytosis in arh^–/– HDFs. (A) Western blot analysis. arh^–/– HDFs were treated with siRNA for Dab2 and CHC, and cell lysates were analyzed by western blot for efficiency of knockdown and total LDLR levels. (B-D) LDLR endocytosis was assessed in arh^–/– fibroblasts as in Fig. 2A-F. Bar, 15 µm. (E) The percent of cells internalizing LDLR was determined in two separate experiments by counting approximately 50 cells of each condition, and these were averaged together. Depletion of either Dab2 or CHC caused a significant block in LDLR endocytosis (**P<0.01).