Fig. 4. Dab2-depletion slows the rate of miniLDLR endocytosis. siRNA-treated HeLa cells were detached from dishes with 10 mM EDTA, and antibody-binding and -internalization experiments were performed on cells in suspension. The mean fluorescence intensity for each population was measured by FACS analysis, and average values and standard errors are shown for three independent experiments. (A) Initial steady-state receptor levels in control versus siRNA-treated cells were compared (P<0.05 as determined by t-test). (B) Receptor endocytosis was followed by monitoring the decrease in surface receptor levels following 0, 5 and 10 minutes at 25°C (*P<0.01 and P<0.05 as determined by t-test).