Fig. 7. Characterization of rosette-like structures in the NE of cPAR3-overexpressing embryos. (A,A') Cycling cells (BrdU+) are present outside the VZ (bars). (B-B'') cPAR3 is localized in a central ring inside the rosettes, together with cPAR6/aPKC. (C,C') Rosette cells (dotted line) contain centrosomes close to the central lumen (dotted circle). (D,D') ß-Catenin (arrowhead) and N-Cadherin (arrowhead in E,E') localize with cPAR3::GFP at the center of rosettes. (F,G) cPAR3 overexpression (left side of the NT) causes an expansion of the cNotch1 and ches5-1 expression domains (white brackets). (H) Ectopic cDelta1 expressing cells can be detected in the basal region of the cPAR3-electroporated NT (white brackets). (I,I') Rosette cells express ches5-1. (J,J') TUJ1- and HU-positive neurons (K,K') accumulate outside rosettes. Magnification in (A,A'), 40x; magnification in (F-H), 20x. Bars in (B-B''), 4 µm; bars in (C-D',J,J'), 10 µm; bars in (I,I'), 5 µm; bars in (K,K'), 20 µm.