Fig. 1. TSA induces cardiomyogenesis. P19 cells were aggregated in the (A,B) absence of DMSO, (C,D) with 0.8% DMSO or (E,F) with 5 nM TSA. After 6 days, cells were stained with an anti-myosin heavy-chain antibody (anti-MyHC) and examined by immunofluorescence (B,D,F). Hoechst dye 33258 was used to visualize nuclei (A,C,E). Magnification 400x.