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Figure 3


Fig. 3. MEF2C/EnR expressed via an Nkx2-5 enhancer abrogated heart muscle development in type-I transgenic mouse embryos. Wild-type (A,D,G,J), type-I (B,E,H,K) and type-II (C,F,I,L) embryos were harvested on E9.5 and photographed under a dissecting microscope (A-C). The heart area is indicated by a circle. Cryosections were prepared and in situ hybridization was performed with probes against Nkx2-5 (D-F), cardiac {alpha}-actin (G-I), and Gata4 (J-L). The heart region (arrowhead) and head (h) are indicated.