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Figure 5


Fig. 5. Myosin, myomesin, obscurin and titin lack ordered organization in homozygous targeted cells. Confocal images of triple-stained homozygous targeted cells. Cells were co-stained for (A) actin (using Alexa-546 phalloidin), the Z-disk protein {alpha}-actinin, and myosin; (B) actin (using Alexa-546 phalloidin) {alpha}-actinin, and the M-band region of titin; (C) the Z-disk epitope of titin, myomesin and DAPI; or (D) myosin, myomesin and obscurin. Arrows in A and B show stripes of {alpha}-actinin staining that might be immature Z-disks and which have a spacing of approximately 1 µm. Arrows in C show close association of titin spots with myomesin. Arrows in D show occasional colocalisation of myomesin and obscurin. Arrowheads show spots of the rare association of myomesin spots with myosin. Bars, 5 µm.