Fig. 3. Role of Arg-based signals in 14-3-3-dependent forward transport. Columns show surface expression of WT and mutant SUR1-Kir6.2-fusion proteins (as indicated) in the absence (black bars) or presence (grey bars) of the 14-3-3-scavenger pGpLI-R18. For each fusion protein, data were obtained from >90 oocytes (from 18 to 22 batches) and were normalized to the values measured in the absence of pGpLI-R18 (see Materials and Methods). Data for SUR1_RKR-Kir6.2_RKR and SUR1_RKR-Kir6.2_AAA were significantly different (P<0.0001) in the presence and absence of pGpLI-R18. Western blots of oocyte homogenates for the same constructs probed with an anti-Kir6.2 antibody are shown underneath the bar graph. For each construct, there was no difference in expression when pGpLI-R18 was present. M indicates molecular mass in kDa.