Fig. 4. Gliotactin somatic clones in the wing disc have a normal distribution of septate junction proteins. (A-F) Somatic clones of Gliotactin null mutant in columnar epithelial cells of the wing disc. (A) Clone labeled with Neurexin IV (green) and Dlg (red); mutant cells lack GFP (blue) to show the location of the clone (outlined). (B,C) Side views to show the normal protein distribution in wild-type versus mutant cells. Dlg (B, red) and GFP (B, blue) or Dlg alone (B'). Neurexin IV (C, green) and GFP (C, blue) or Neurexin IV alone (C'). The dashed lines indicate the position of Gliotactin null cells. (D) Dlg (red channel from A) shown in grayscale. Arrowheads indicate the location of the TCJ. (E) Neurexin IV (green channel from A) shown in grayscale. (F) Somatic clones of Gliotactin showing the absence of Gliotactin staining in null clones. (G-I) Somatic clones of Gliotactin mutant cells in peripodial cells of the wing imaginal disc. (G) A clone labeled with Dlg shows normal distribution of Dlg at the TCJ. Compare arrowheads indicating the TCJ in the clone with wild-type cells. (H) Clone of Gliotactin mutant cells labeled with Neuroglian. (I) Clone labeled with E-cadherin. Bars, 15 µm (A-E, G-I); 40 µm (F).