Fig. 6. Ectopic expression of Gliotactin in wing imaginal discs. (A,A') Patched-GAL4 driving full-length Gliotactin. (A) When Gliotactin (green) is overexpressed it is found throughout the cell membrane. There is a reduction in Dlg (red) staining intensity compared with neighbouring wild-type cells. (A') At higher magnification, the Gliotactin overexpressing cells adjacent to wild-type cells clearly show the reduced levels of Dlg compared with non-expressing cells (arrows). The Gliotactin channel (green) was collected at saturating levels of Gliotactin so that the boundary cells could be better assayed. A' has been digitally scaled to 200% of A. (B,B') Apterous-GAL4 driven overexpression of full length Gliotactin. (B) Overexpression of Gliotactin (green) with apterous-GAL4 also results in a reduction of Dlg staining (red). (B') A longitundinal x-axis projection of Gliotactin (red) and Dlg (green) and panels showing each individual channel in the LUT scale to indicate intensity of staining. Overexpressed Gliotactin is throughout the cell membrane with the greatest concentration around the apical/SJ region. Dlg expression in these regions is reduced throughout the cell and at the SJ (arrow). The Dlg levels in the overlying peripodial cell layer are not affected (arrowhead) because this cell layer does not express apterous-GAL4. The fire LUT scale ranges from purple (lowest) to white (highest) intensity. (C) Overexpression of GliotactinPDZ (green) has no effect on Dlg (red) levels. GliotactinPDZ spreads around the cell but fails to downregulate Dlg. (D) Overexpression of full-length Gliotactin (green) had no effect on Coracle (red) levels. (E-F) Overexpression of Gliotactin in embryonic epidermis using 69B-GAL4. (E) Overexpression of full-length Gliotactin (green) had little effect on Dlg (red) levels. (F) Similarly, overexpression of GliotactinPDZ (green) had little effect on Dlg (red). Bars, 15 µm.