Fig. 5. Mislocalization of RacGAP50C causes ectopic furrowing. Expression of CD8::GFP and CD8::GFP::RacGAP50C was induced by incubating the cultures in complete medium containing 0.7 mM CuSO₄ for 5-7 hours. The cells were then fixed and stained to reveal GFP (green in the merged panels), DNA (blue in the merged panels) and either tubulin (A), Anillin (B), Peanut (C) or PAV (D) (red in the merged panels). The arrows indicate the ectopic furrows whereas the arrowhead indicates the equatorial furrow. Note the misshapen and bisected nuclei in A and B and the small cytoplast in C. Bars, 10 µm.