Fig. 6. The membrane-tethered version of RacGAP50C can induce ectopic furrows in the absence of PAV. CD8::GFP and CD8::GFP::RacGAP50C cells were incubated with dsRNA directed against PAV for 48 hours and then the expression of transgenes induced by incubating the cultures in complete medium containing 0.7 mM CuSO4 for 5-7 hours. The cells were then fixed and stained to reveal GFP (green in the merged panels), tubulin (red in the merged panels) and DNA (blue in the merged panels). The arrows mark the ectopic furrows. Note that the size of the nuclei of the CD8::GFP::RacGAP50C cell shown at the bottom indicates that this cell is tetraploid and therefore already failed cytokinesis once. Nonetheless, multiple ectopic furrows still formed and CD8::GFP::RacGAP50C co-localized with the remnant of the central spindle. Bar, 10 µm.