Fig. 1. Drosophila Nup214 is in complex with Nup88 and CRM1. The amounts of CRM1 bound to the Nup214-Nup88 complex vary during fly development. Nup214 antiserum was used to coimmunoprecipitate protein complexes of 0-5, 5-10 and 10-15 hour wild-type embryo extracts. Extracts, unbound fractions and precipitates (IP) were analyzed by labeling the western blots with Nup214, CRM1 and Nup88 antibodies. Lamin serves as a loading control. Total amounts of Nup214, Nup88 and CRM1 appear unchanged in the extracts from different embryonic stages. The amount of co-immunoprecipitated CRM1 relative to Nup214 does not change in 0-5 hour and 5-10 hour embryos whereas three times less CRM1 is brought down in 10-15 hour embryos.