Fig. 2. Nup88 is degraded in nup214 mutants. (A) Nup214, mAb414, lamin and Nup88 localization (red) in fat body cells of wild-type and nup214 mutant larvae. Nuclei are visualized by DAPI in the adjacent panel. Confocal sections are shown in the insets. Bars, 35 µm. (B) Western blot of protein extract from wild-type, heterozygotes and nup214 mutant larvae probed with Nup88 antibody. ß-tubulin provides a loading control. Nup88 is reduced in heterozygote animals and totally abolished in the homozygous mutant. (C) RT-PCR for mbo on poly(A)⁺-purified larval extracts of wild-type (lanes 1-4), P-element (l(2)10444) (lanes 5-8) and nup214 mutants (lanes 9-12). (Fourfold serial dilutions of mRNA from each genotype were used in the RT-PCR step.) rp49 provides a quantitative control. The mRNA levels of mbo remain the same in wild-type and mutant larvae.