Fig. 8. Co-expression of Nup214 and Nup88 in the wild type is sufficient to mislocalize endogenous targets. Nup214 and Nup88 (HS214+HS88) expression was driven by the hsp70 promoter in wild-type third instar larvae. Heat-treated wild-type (wt) served as a control. (A) Overexpression of Nup214 and Nup88 interferes with the expression of a drosomycin-GFP (dromGFP) reporter. All panels show fat bodies, before (not challenged) and after bacterial infection (bact. challenged) stained with GFP antibody. The images for HS214+HS88 unchallenged and infected larval fat bodies were acquired at three times lower intensity for Nup214 labeling compared with the wild type. (B) Confocal sections of malpighian tube nuclei stained with Nup88, Nup214, Dorsal, CRM1 or Grainyhead antiserum. Co-expression of Nup214 and Nup88 can mislocalize Dorsal but not Grainyhead. Bars, 35 µm (A); 5 µm (B).