Fig. 1. p150Glued antibodies. (A) S2 cell extracts were probed with pre-immune (PI), immune (I) sera Rb1477 (left), Rb1478 (middle) or affinity-purified antibodies (right, AP). The position of the p150Glued protein is indicated with the arrowhead. (B) Extracts from S2 cells treated (+) or not () with Glued dsRNA were analysed with CP190, aurora A, cyclin B and dynein intermediate chain (DIC) antibodies (left) as loading controls, as well as with the affinity-purified anti-p150Glued antibodies (right).