Fig. 2. p150^Glued localises to centrosomes, spindle and interphase microtubules. S2 cells (A and B) or early Drosophila syncitial embryos (C) were fixed and stained with the affinity-purified anti-p150^Glued antibodies (red and lower panels in monochrome), the anti--tubulin antibody (green and middle panels in monochrome) and DAPI (DNA staining in blue). (A) Mitotic cells. (B) Interphase cells: the right column shows a 10x magnified view of the border of a flat S2 cell. Note the punctuate staining along microtubule fibres. a, anaphase; c, cytokinesis; m, metaphase; p, prophase; t, telophase. (D) A mitotic cell treated with colchicine to depolymerise the mitotic spindle was stained for polo kinase (red and upper right panel in monochrome) and p150^Glued (green and lower panel on the right in monochrome). The lower left panel shows a 10x magnification view of the kinetochore region boxed in the panel above. Bars, 10 µm (A-C); 1 µm (D).