Fig. 3. Glued RNAi leads to anaphase defects. Control (A) or Glued dsRNA-treated (B) mitotic S2 cells were fixed and stained for -tubulin (red), -tubulin (green and middle panels in monochrome) and DNA (blue and lower panels in monochrome). a, anaphase; c, cytokinesis; m, metaphase; p, prophase; t, telophase. In p150^Glued-depleted cells, cells are delayed in metaphase (see also Table 1). 50% of these cells show weak centrosome connection to spindle poles (panel 2) whereas others show a normal shape (panel 1). Note the segregation defect and the absence of microtubule bundling during anaphase (panels 3 and 4). During telophase, a central spindle forms but with strongly disorganised microtubules and a lack of well-defined microtubule bundles (panel 5). (C) FACS analysis of Glued dsRNA-treated cells does not show any accumulation of polyploid cells but evidences an increase of apoptotic or aneuploid cells (arrow) compared with control cells. Bar, 10 µm.