Fig. 7. Pavarotti-KLP targeting to the plus ends of microtubules and central spindle formation is delayed in Glued dsRNA-treated cells. Control or Glued dsRNA-treated mitotic cells were stained for Pavarotti-KLP (red and lower panels in monochrome), DNA (blue) and microtubules (green). (A) Pavarotti-KLP faintly localises to the mitotic spindle during metaphase (panel 1) and strongly accumulates at the plus ends of microtubules during anaphase (panel 2), telophase (panel 3) and the midbody during cytokinesis (panel 4). (B) Pavarotti-KLP localises to the mitotic spindle in a p150^Glued knockdown metaphase cell (panel 1) but is not relocalised to microtubule plus ends during anaphase and transiently accumulates at the cell periphery where cortex contractions are detected (panel 2). As the cell progresses through anaphase-B-telophase, Pavarotti-KLP starts to accumulate on the microtubules present between the two chromatid masses (panel 3) allowing a reduced central spindle to form (panel 4) and cytokinesis to occur (panel 5). Bar, 10 µm.