Fig. 7. Biochemical characterization of exosomes derived from K562 and HepG2 cells. Equal protein amounts of exosomes (EXO) and LDTI (left panel) or total cellular lysates (right panel) obtained from K562 wt, K562 cav and HepG2 cells were resolved by SDS-PAGE and immunoblotted with the use of antibodies for TfR2, CD81, TfR1, caveolin-1, the raft-marker Lyn, the exosomal-lysosomal marker Lamp-2, and actin to demonstrate equivalent protein loading and transfer.