Fig. 4. NoC1 binding to versican. Binding of [¹²⁵I]-NoC1 to wells coated with the indicated concentrations of full-length versican (A) or VG1 (B) in buffers containing Ca²⁺ and Mg²⁺ () or 5 mM EDTA () for 3 hours at 37°C. Background values were subtracted from each data point (365.8±28.7 and 286.0±21.3). (C) Binding of [¹²⁵I]-TSP1 to wells coated using 1 µg per well of versican () or 0.3 µg per well of VG1 () was determined in the presence of the indicated concentrations of heparin. Binding was normalized to that determined in the absence of heparin (748.8±24.2 and 12658.8±117.4 cpm, respectively). All data are mean ± s.e.m. for quadruplicate determinations.