Fig. 8. High concentrations of exogenous TSP1 inhibit inflammation-induced extracellular deposition of versican and elastin. HASMC were cultured without (control) or with 5 µg ml^–1 poly I:C for 4 hours in the presence or absence of 20 µg ml^–1 TSP1. Cells were subsequently fixed and double stained as indicated in the Materials and Methods. Exogenous TSP1 diminished versican and elastin accumulation, but not hyaluronan, as seen by double immunofluorescence staining for TSP1 (red), for versican, elastin or hyaluronan (green) and nuclei (blue). Bars, 50 µm.