Fig. 9. Heparin inhibits TLR3-induced deposition of exogenous TSP1. HASMC were cultured in standard medium (control) or in the presence of 5 µg ml^–1 poly I:C for 15 hours, followed by an additional 1 hour incubation in culture medium containing 10 µg ml^–1 TSP1 with or without the indicated concentrations of heparin. Staining with antibody against TSP1 showed that binding of exogenous protein to the structures generated by cells following poly I:C treatment was inhibited by heparin in a dose-dependent manner. Bar, 50 µm.