Fig. 4. Fluorescence images of stripped capsules with attached lens epithelial cells from 6-week-old nestin-cre positive Cx43+/flox (A,B) and Cx43flox/flox (C,D) lenses. Cx43 is stained with the Chemicon anti-Cx43 reagent (A,C) and nuclei are visualized with Hoechst staining (B,D). There is a loss of the punctate gap junctional staining in the homozygote (C) demonstrating loss of Cx43 from the lens epithelium.