Fig. 2. Nef modulates cytokine and growth factor production in astrocytes. U251MG-Nef_Bru-4/4.2 and U251MG-parental cells were incubated in VLE-RPMI1640, 0.5% FCS for 16 hours. Supernatants were analyzed with RayBio® Human Cytokine antibody array V. (A) Scheme of the spotted primary antibodies. (B) Images after fluorescence scanning. (C) List of differentially regulated proteins. The ratios indicated are calculated by using the fluorescence intensities of the corresponding protein spots after background (Neg) correction and normalization of the intensities according to the mean intensities of the positive controls (Pos).