Fig. 3. Localization of the a3 isoform to secretory granules in ßTC6 and ßHC9 cells. (A) ßTC6 cells grown on a slide glass were stained with antibodies against the a3 isoform (green), and insulin, lamp-2, GM130, or synaptophysin (red). (B) ßHC9 cells were stained with antibodies against the a isoforms (a1, a2 and a3, green) and insulin, lamp-2, GM130, or synaptophysin (red). Merged images are also shown. Bar, 10 µm.